Technical support epitope designing and antigen selection. Our Antibody response rate is very high.
Monoclonal antibody development protocol: Standard protocol:
| Antigen |
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To be supplied by customer |
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| Peptide design & synthesis |
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If the antigen is not available or difficult to purify |
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| Conjugation |
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Conjugation of peptide antigens to carrier protein
(KLH or BSA) |
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| Phase I (Immunization) |
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Immunization of 5 Balb/c mice per antigen
Circulating antibody titers tested by ELISA |
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| Phase II (Fusion) |
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Fusion of splenocytes with mouse myeloma cell line (SP2/O). |
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| Phase III (Screening) |
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Primary screening of fusion products by ELISA |
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| Phase IV (Cloning) |
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Positive clones identified as parent clones and then sub cloned by sub limiting dilution. |
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| Phase V (Finalization) |
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Establishment of monoclonality, stabilization and expansion of clones. |
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| Phase VI (Shipment) |
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Shipment of best 3 hybridoma clones to customer's
lab or storage at our facility for continuous supply |
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- Deliverables: 3 best hybridoma clones.
- Turn around time: 4- 5 months. (Till the final hybridoma stage)
Monoclonal antibody development price:
We offer our Services at an extremely attractive prices and offer the Customers the flexibility to pay against milestones achieved.
Contact us for our attractive prices.
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