Polyclonal antibody development in rabbits, goats & sheep
Polyclonal antibody development protocol
| Step 1 |
: |
Peptide conjugation to carrier protein (If the antigen is peptide) |
| Step 2 |
: |
Pre-immune serum collection |
| Step 3 |
: |
Primary injection in Freund’s Complete Adjuvant (FCA) |
| Step 4 |
: |
First booster in Freund’s Incomplete Adjuvant (FIA) |
| Step 5 |
: |
Second booster in Freund’s Incomplete Adjuvant (FIA) |
| Step 6 |
: |
Test bleed 10 days after 2nd booster injection |
| Step 7 |
: |
ELISA screening to check circulating antibody titers |
| Step 8 |
: |
Third booster in Freund’s Incomplete Adjuvant (FIA) |
| Step 9 |
: |
Serum collection |
| Step 10 |
: |
Final bleeding by veterinarian |
| Step 11 |
: |
ELISA screening of final bleed |
- Deliverables: 50 – 60 ml high titer immune serum from both rabbits. (We also give 1 ml pre immune serum per rabbit for use as a negative control in customer assays)
- Turn around time: 2-3 months.
We can maintain the animals on a long term basis (at a reasonable price) if the customer requires continuous supply of the high titre immune serum.
Polyclonal antibody development price:
We offer our Services at an extremely attractive prices and offer the Customers the flexibility to pay against milestones achieved.
Contact us for our attractive prices.
|
