nano flow anti troponin

nano flow anti troponin

DESCRIPTION

General description

Troponin T1, slow skeletal type (TNNT1) is encoded by the gene mapped to human chromosome 19q13.4. The encoded protein is composed of 261 amino acids and is expressed specifically in slow skeletal muscle.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Troponin T plays a vital role in calcium regulation of striated muscle contraction and relaxation. The alternative splicing regulation of troponin T1, slow skeletal type (TNNT1) expression has been implicated in regulation of muscle contractility in physiological and pathophysiological adaptations. Mutation in the gene is associated with the development of Nemaline myopathy.
Performance of nanoprobes can often determine the detection level of Lateral immunochromatography. Traditional probes were limited by the quantity and orientation of antibodies, immune activity of the Fab region or binding strength between protein and substrate. This study developed a new efficient and robust technology to construct fluorescent nanoprobes with oriented modified antibodies, based on specific binding of the Fc region of antibody with streptococcal protein G (SPG) on the surface of polystyrene microspheres (MS) and subsequent covalent cross-linking at binding sites to firm them. Lateral flow immunoassay using these probes was applied for the detection of cardiac troponin I (cTnI). The significantly improved detection sensitivity demonstrated that antibody orientation on MS surfaces effectively enhanced immunological activities of probes compared with random immobilizing methods. Furthermore, performance evaluation results of lateral flow test strips met clinical requirements perfectly, including limit of detection (0.032 ng/mL), linearity (R > 0.99), repeatability (CV < 10%), correlation (R > 0.99), and heat aging stability. This research also employed heterophilic blocking reagent (HBR) to actively block redundant binding sites of SPG for the first time in order to eliminate false positive interferences, improving the sensitivity and precision of test results further.
nano flow anti troponin
nano flow anti troponin
The lateral flow immunoassay (LFIA) has emerged as a powerful tool for rapid screening owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-enhanced Raman scattering LFIA based on nonspherical gap-enhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform SERS response, an order of magnitude higher than that of other common SERS tags such as Au nanorods, nanostars, Au nanoshells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was demonstrated by the semiquantitative and sensitive detection of the heart disease biomarker cardiac troponin I (cTnI). GERTs were conjugated with monoclonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presence of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.

anti-Cardiac Troponin T

YF-PA15080 100 ug
EUR 483.6
Description: Rabbit polyclonal to Cardiac Troponin T

anti-cardiac Troponin C

YF-PA24871 50 ul
EUR 400.8
Description: Mouse polyclonal to cardiac Troponin C

anti-Cardiac Troponin I

YF-PA24873 50 ul
EUR 400.8
Description: Mouse polyclonal to Cardiac Troponin I

Anti-Cardiac Troponin I (1E7)

YF-MA10951 100 ug
EUR 435.6
Description: Mouse monoclonal to Cardiac Troponin I

anti- Cardiac Troponin I antibody

FNab01273 100µg
EUR 658.5
Description: Antibody raised against Cardiac Troponin I

anti- Cardiac Troponin T antibody

FNab01274 100µg
EUR 658.5
Description: Antibody raised against Cardiac Troponin T

anti- Cardiac Troponin T antibody

FNab09914 100µg
EUR 658.5
Description: Antibody raised against Cardiac Troponin T

anti- Cardiac Troponin I  antibody

FNab09781 100µg
EUR 658.5
Description: Antibody raised against Cardiac Troponin I 

Anti-TNNI3/Troponin I Antibody

A01720-1 100ul
EUR 476.4
Description: Rabbit Polyclonal TNNI3/Troponin I Antibody. Validated in IF, IHC, WB and tested in Mouse, Rat.

anti- Cardiac Troponin T antibody

LSMab09914 100 ug
EUR 463.2

Anti-Cardiac Troponin T Antibody

ER21102 100ul
EUR 189
Description: Troponin T type 2 (cardiac), also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations of this gene may be associated with mild or absent hypertrophy and predominant restrictive disease, with a high risk of sudden cardiac death.

Anti-Cardiac Troponin I antibody

PAab01273 100 ug
EUR 463.2

Anti-Cardiac Troponin T antibody

PAab01274 100 ug
EUR 463.2

Anti-cardiac troponin T antibody

STJ72901 100 µg
EUR 430.8

Anti-Cardiac Troponin I Antibody

24001 100ug
EUR 235

Anti-Cardiac Troponin I Antibody

24002 100ug
EUR 235

Anti-Cardiac Troponin T Antibody

24101 100ug
EUR 235

Anti-Cardiac Troponin T Antibody

24102 100ug
EUR 235

Anti-cardiac Troponin C (1F8-A9)

YF-MA10950 100 ug
EUR 435.6
Description: Mouse monoclonal to cardiac Troponin C

Anti-Cardiac Troponin T (3H4-F7)

YF-MA15918 100 ug
EUR 435.6
Description: Mouse monoclonal to Cardiac Troponin T

Canine Troponin T, Troponin T GENLISA ELISA

KLN0221 1 x 96 wells
EUR 341

Anti-Cardiac Troponin T (cTnT) Antibody

Y059696 100 µg
EUR 425

Goat anti-cardiac troponin T Antibody

dAP-2743 50ug
EUR 264.6

Anti- Troponin I (cardiac) (88-106) Antibody

GWB-3A08FD 1 mg Ask for price

Anti- Troponin I (cardiac) (88-106) Antibody

GWB-98BF5C 1 mg Ask for price

Anti- Troponin I (cardiac) (27-40) Antibody

GWB-9F31FC 1 mg Ask for price

Anti- Troponin I (cardiac) (27-40) Antibody

GWB-933657 1 mg Ask for price

Anti-Cardiac Troponin T/TNNT2 Antibody

A01154-1 100ug/vial
EUR 352.8

Anti-cardiac Troponin C/TNNC1 Antibody

PA1779 100ug/vial
EUR 400.8

Rabbit anti Troponin I Polyclonal Antibody

TA354523 100 µg Ask for price

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