Performance of nanoprobes can often determine the detection level of Lateral immunochromatography. Traditional probes were limited by the quantity and orientation of antibodies, immune activity of the Fab region or binding strength between protein and substrate. This study developed a new efficient and robust technology to construct fluorescent nanoprobes with oriented modified antibodies, based on specific binding of the Fc region of antibody with streptococcal protein G (SPG) on the surface of polystyrene microspheres (MS) and subsequent covalent cross-linking at binding sites to firm them. Lateral flow immunoassay using these probes was applied for the detection of cardiac troponin I (cTnI). The significantly improved detection sensitivity demonstrated that antibody orientation on MS surfaces effectively enhanced immunological activities of probes compared with random immobilizing methods. Furthermore, performance evaluation results of lateral flow test strips met clinical requirements perfectly, including limit of detection (0.032 ng/mL), linearity (R > 0.99), repeatability (CV < 10%), correlation (R > 0.99), and heat aging stability. This research also employed heterophilic blocking reagent (HBR) to actively block redundant binding sites of SPG for the first time in order to eliminate false positive interferences, improving the sensitivity and precision of test results further.

The lateral flow immunoassay (LFIA) has emerged as a powerful tool for rapid screening owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-enhanced Raman scattering LFIA based on nonspherical gap-enhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform SERS response, an order of magnitude higher than that of other common SERS tags such as Au nanorods, nanostars, Au nanoshells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was demonstrated by the semiquantitative and sensitive detection of the heart disease biomarker cardiac troponin I (cTnI). GERTs were conjugated with monoclonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presence of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.

anti-Cardiac Troponin I
YF-PA15073	Abfrontier	50 ul	EUR 435.6
Description: Mouse polyclonal to Cardiac Troponin I

anti-Cardiac Troponin I
YF-PA15074	Abfrontier	100 ug	EUR 483.6
Description: Rabbit polyclonal to Cardiac Troponin I

anti-Cardiac Troponin T
YF-PA15078	Abfrontier	50 ug	EUR 435.6
Description: Mouse polyclonal to Cardiac Troponin T

anti-Cardiac Troponin T
YF-PA15079	Abfrontier	100 ul	EUR 483.6
Description: Rabbit polyclonal to Cardiac Troponin T

anti-Cardiac Troponin T
YF-PA15080	Abfrontier	100 ug	EUR 483.6
Description: Rabbit polyclonal to Cardiac Troponin T

Mouse anti Troponin T (Cardiac)
MBS225448-INQUIRE	MyBiosource	INQUIRE	Ask for price

Mouse anti Troponin I (Cardiac)
MBS225486-INQUIRE	MyBiosource	INQUIRE	Ask for price

Rabbit anti Troponin I antibody
MBS462147-01mg	MyBiosource	0.1mg	EUR 300

Rabbit anti Troponin I antibody
MBS462147-5x01mg	MyBiosource	5x0.1mg	EUR 1310

Rabbit anti Troponin I antibody
MBS462149-01mg	MyBiosource	0.1mg	EUR 320

Rabbit anti Troponin I antibody
MBS462149-5x01mg	MyBiosource	5x0.1mg	EUR 1390

MOUSE ANTI CHICKEN TROPONIN T
MBS213031-025mg	MyBiosource	0.25mg	EUR 530

MOUSE ANTI CHICKEN TROPONIN T
MBS213031-5x025mg	MyBiosource	5x0.25mg	EUR 2215

Anti-Cardiac Troponin I (1E7)
YF-MA10951	Abfrontier	100 ug	EUR 435.6
Description: Mouse monoclonal to Cardiac Troponin I

Anti-TNNI3/Troponin I Antibody
A01720-1	BosterBio	100ul	EUR 335
Description: Boster Bio Anti-TNNI3/Troponin I Antibody (Catalog# A01720-1). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Mouse, Rat.

anti- Cardiac Troponin I  antibody
FNab09781	FN Test	100µg	EUR 658.5
Description: Antibody raised against Cardiac Troponin I

anti- Cardiac Troponin T antibody
FNab09914	FN Test	100µg	EUR 658.5
Description: Antibody raised against Cardiac Troponin T

anti- Cardiac Troponin I antibody
LSMab09781	Nova Lifetech	100ug	EUR 325

anti- Cardiac Troponin T antibody
LSMab09914	Lifescience Market	100 ug	EUR 463.2

anti- Cardiac Troponin I antibody
FNab01273	FN Test	100µg	EUR 658.5
Description: Antibody raised against Cardiac Troponin I

anti- Cardiac Troponin T antibody
FNab01274	FN Test	100µg	EUR 658.5
Description: Antibody raised against Cardiac Troponin T

anti-Human Cardiac Troponin I (24-40)
MBS561009-1mg	MyBiosource	1mg	EUR 395

anti-Human Cardiac Troponin I (24-40)
MBS561009-5x1mg	MyBiosource	5x1mg	EUR 1630

anti-Human Cardiac Troponin I (41-49)
MBS561011-1mg	MyBiosource	1mg	EUR 395

anti-Human Cardiac Troponin I (41-49)
MBS561011-5x1mg	MyBiosource	5x1mg	EUR 1630

anti-Human Cardiac Troponin I (41-49)
MBS561012-1mg	MyBiosource	1mg	EUR 395

anti-Human Cardiac Troponin I (41-49)
MBS561012-5x1mg	MyBiosource	5x1mg	EUR 1630

Anti-Cardiac Troponin I Antibody
24001	Genovis AB	100ug	EUR 235

Anti-Cardiac Troponin I Antibody
24002	Genovis AB	100ug	EUR 235

Anti-Cardiac Troponin T Antibody
24101	Genovis AB	100ug	EUR 235