nano flow anti troponin
- Lieven
- 0
DESCRIPTION
General description
Troponin T1, slow skeletal type (TNNT1) is encoded by the gene mapped to human chromosome 19q13.4. The encoded protein is composed of 261 amino acids and is expressed specifically in slow skeletal muscle.
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Troponin T plays a vital role in calcium regulation of striated muscle contraction and relaxation. The alternative splicing regulation of troponin T1, slow skeletal type (TNNT1) expression has been implicated in regulation of muscle contractility in physiological and pathophysiological adaptations. Mutation in the gene is associated with the development of Nemaline myopathy.
Performance of nanoprobes can often determine the detection level of Lateral immunochromatography. Traditional probes were limited by the quantity and orientation of antibodies, immune activity of the Fab region or binding strength between protein and substrate. This study developed a new efficient and robust technology to construct fluorescent nanoprobes with oriented modified antibodies, based on specific binding of the Fc region of antibody with streptococcal protein G (SPG) on the surface of polystyrene microspheres (MS) and subsequent covalent cross-linking at binding sites to firm them. Lateral flow immunoassay using these probes was applied for the detection of cardiac troponin I (cTnI). The significantly improved detection sensitivity demonstrated that antibody orientation on MS surfaces effectively enhanced immunological activities of probes compared with random immobilizing methods. Furthermore, performance evaluation results of lateral flow test strips met clinical requirements perfectly, including limit of detection (0.032 ng/mL), linearity (R > 0.99), repeatability (CV < 10%), correlation (R > 0.99), and heat aging stability. This research also employed heterophilic blocking reagent (HBR) to actively block redundant binding sites of SPG for the first time in order to eliminate false positive interferences, improving the sensitivity and precision of test results further.
The lateral flow immunoassay (LFIA) has emerged as a powerful tool for rapid screening owing to its simplicity and flexibility for detection of various biomarkers. However, conventional LFIA strips have several disadvantages, including limits in quantitative analysis and low sensitivity. Here we developed a novel surface-enhanced Raman scattering LFIA based on nonspherical gap-enhanced Raman tags (GERTs), with Raman molecules (RMs) embedded in a 1-nm gap between Au nanorod core and Au shell. Such tags have a strong and uniform SERS response, an order of magnitude higher than that of other common SERS tags such as Au nanorods, nanostars, Au nanoshells with surface-adsorbed RMs, or spherical GERTs with embedded RMs. The feasibility of the tags was demonstrated by the semiquantitative and sensitive detection of the heart disease biomarker cardiac troponin I (cTnI). GERTs were conjugated with monoclonal antibodies and used for LFIA in the same way as ordinary functionalized colloidal gold. The presence of the target antigen, cTnI, was identified by Raman microscopy mapping of the test zone. With the SERS-based LFIA, the limit of cTnI detection was about 0.1 ng/mL. This value is within the diagnostic range of cTnI in the blood serum of patients with heart infarction and is 30 times lower than that of the colorimetric LFIA test using the same antibodies and either GERTs or colloidal gold as labels.
anti-Cardiac Troponin T |
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YF-PA15080 | Abfrontier | 100 ug | EUR 483.6 |
Description: Rabbit polyclonal to Cardiac Troponin T |
anti-cardiac Troponin C |
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YF-PA24871 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to cardiac Troponin C |
anti-Cardiac Troponin I |
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YF-PA24873 | Abfrontier | 50 ul | EUR 400.8 |
Description: Mouse polyclonal to Cardiac Troponin I |
Anti-Cardiac Troponin I (1E7) |
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YF-MA10951 | Abfrontier | 100 ug | EUR 435.6 |
Description: Mouse monoclonal to Cardiac Troponin I |
anti- Cardiac Troponin I antibody |
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FNab01273 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against Cardiac Troponin I |
anti- Cardiac Troponin T antibody |
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FNab01274 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against Cardiac Troponin T |
anti- Cardiac Troponin T antibody |
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FNab09914 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against Cardiac Troponin T |
anti- Cardiac Troponin I antibody |
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FNab09781 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against Cardiac Troponin I |
Anti-TNNI3/Troponin I Antibody |
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A01720-1 | BosterBio | 100ul | EUR 476.4 |
Description: Rabbit Polyclonal TNNI3/Troponin I Antibody. Validated in IF, IHC, WB and tested in Mouse, Rat. |
anti- Cardiac Troponin T antibody |
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LSMab09914 | Lifescience Market | 100 ug | EUR 463.2 |
Anti-Cardiac Troponin T Antibody |
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ER21102 | HUABIO | 100ul | EUR 189 |
Description: Troponin T type 2 (cardiac), also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations of this gene may be associated with mild or absent hypertrophy and predominant restrictive disease, with a high risk of sudden cardiac death. |
Anti-Cardiac Troponin I antibody |
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PAab01273 | Lifescience Market | 100 ug | EUR 463.2 |
Anti-Cardiac Troponin T antibody |
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PAab01274 | Lifescience Market | 100 ug | EUR 463.2 |
Anti-cardiac troponin T antibody |
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STJ72901 | St John's Laboratory | 100 µg | EUR 430.8 |
Anti-Cardiac Troponin I Antibody |
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24001 | Genovis AB | 100ug | EUR 235 |
Anti-Cardiac Troponin I Antibody |
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24002 | Genovis AB | 100ug | EUR 235 |
Anti-Cardiac Troponin T Antibody |
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24101 | Genovis AB | 100ug | EUR 235 |
Anti-Cardiac Troponin T Antibody |
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24102 | Genovis AB | 100ug | EUR 235 |
Anti-cardiac Troponin C (1F8-A9) |
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YF-MA10950 | Abfrontier | 100 ug | EUR 435.6 |
Description: Mouse monoclonal to cardiac Troponin C |
Anti-Cardiac Troponin T (3H4-F7) |
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YF-MA15918 | Abfrontier | 100 ug | EUR 435.6 |
Description: Mouse monoclonal to Cardiac Troponin T |
Canine Troponin T, Troponin T GENLISA ELISA |
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KLN0221 | Krishgen | 1 x 96 wells | EUR 341 |
Anti-Cardiac Troponin T (cTnT) Antibody |
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Y059696 | ABM | 100 µg | EUR 425 |
Goat anti-cardiac troponin T Antibody |
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dAP-2743 | Angio Proteomie | 50ug | EUR 264.6 |
Anti- Troponin I (cardiac) (88-106) Antibody |
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GWB-3A08FD | GenWay Biotech | 1 mg | Ask for price |
Anti- Troponin I (cardiac) (88-106) Antibody |
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GWB-98BF5C | GenWay Biotech | 1 mg | Ask for price |
Anti- Troponin I (cardiac) (27-40) Antibody |
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GWB-9F31FC | GenWay Biotech | 1 mg | Ask for price |
Anti- Troponin I (cardiac) (27-40) Antibody |
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GWB-933657 | GenWay Biotech | 1 mg | Ask for price |
Anti-Cardiac Troponin T/TNNT2 Antibody |
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A01154-1 | BosterBio | 100ug/vial | EUR 352.8 |
Anti-cardiac Troponin C/TNNC1 Antibody |
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PA1779 | BosterBio | 100ug/vial | EUR 400.8 |
Rabbit anti Troponin I Polyclonal Antibody |
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TA354523 | Origene Technologies GmbH | 100 µg | Ask for price |
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