SCL Biosciences

Abstract. The methods which are used to combat pathogens of crops, in particular, chemical
treatment, pose a threat to environmental safety. RNA interference could be one of the best
ways to selectively control pests, but there are not enough methods for introducing dsRNA into
plants without creating GMOs. The development of new methods of dsRNA delivery requires
affordable and fast gene model systems, the purpose of which is our work. We selected the
genes of several carbonic anhydrases functioning in the thylakoids and mitochondria of the
whole plant, the suppression of which will be seen phenotypically based on the data on mutants
for these genes. Specific primers have been designed, and a PCR reaction has also been carried
out to obtain the product of the corresponding mRNA, which will be delivered to the plant in
the future using the VIGS system.

1. Introduction
Agriculture faces a number of challenges, such as insect, disease and weed damage, that decrease
productivity and keep farmers from reaching their potential maximum harvest. In an effort to reduce
pest damage, manufacturers rely heavily on synthetic chemicals. Pesticides have allowed farmers to
increase production, improve product quality and increase profits. However, the large number of
chemicals used every year lead to an increase in pest resistance to the drugs used, and also greatly
affect the environmental situation in the world. Against the background of such a situation, alternative
methods of combating pathogens are acquiring special significance. One of such methods can be the
approaches based on RNA interference.
RNA interference was discovered in 1998 as an effect of suppressing the manifestation of the
genotype when dsRNA was introduced into the body [1]. Later, it became known that RNA silencing
is a part of a complex network of interconnected pathways for cellular defense and regulation of
genes, which can make it a powerful tool for the experimental manipulation of the expression of
individual genes [2]. It can be used to suppress genes for susceptibility to pathogens in agricultural
crops.
In plants, virus induced gene silencing (VIGS) has shown reliable results in suppressing genes,
which uses modified viruses such as the tobacco rattle virus (TRV) to deliver dsRNA into the plant
cell [3]. Since the delivery of viral particles occurs using bacteria of the genus Agrobacterium, random
modifications occur in the plant genome [4-5]. Because of this, accidentally genetically modified
plants cannot be used in agriculture, which means that this method, despite its simplicity, is prohibited
for the use in treating plants from pests or to combat abiotic stress.

AMV Reverse Transcriptase
105-400	GeneOn	200 units	EUR 75.6

AMV Reverse Transcriptase
105-410	GeneOn	1000 units	EUR 216

HIV Reverse Transcriptase
DAG1875	Creative Diagnostics	200 Units	EUR 721.88
Description: Recombinant

HIV Reverse Transcriptase
E1373-01	EURx	500U	EUR 323.73
Description: RNA dependent DNA polymerase from Human Immunoeficiency Virus

HIV Reverse Transcriptase
E1373-02	EURx	2500U	EUR 1294.92
Description: RNA dependent DNA polymerase from Human Immunoeficiency Virus

Tth Reverse Transcriptase
E1374-01	EURx	100U	EUR 86.11
Description: RNA dependent DNA polymerase from Thermus thermophilus, active at elevated temperatures

Tth Reverse Transcriptase
E1374-02	EURx	500U	EUR 345.53
Description: RNA dependent DNA polymerase from Thermus thermophilus, active at elevated temperatures

Evo? Reverse Transcriptase
M1173-100	Biovision	each	EUR 385.2

Evo? Reverse Transcriptase
M1173-25	Biovision	each	EUR 333.6

AMV Reverse Transcriptase
MBT073-100U	EWC Diagnostics	1 unit	EUR 52.39
Description: AMV Reverse Transcriptase

AMV Reverse Transcriptase
MBT073-250U	EWC Diagnostics	1 unit	EUR 125.46
Description: AMV Reverse Transcriptase

RTv Reverse Transcriptase
RV101-01-1500U	Vazyme	1,500 U	EUR 160.78

Novo? Reverse Transcriptase
M1174-100	Biovision	each	EUR 457.2

Novo? Reverse Transcriptase
M1174-25	Biovision	each	EUR 385.2

smART Reverse Transcriptase
E1376-01	EURx	10000U	EUR 71.94
Description: Recombinant reverse transcriptase with reduced RNase H activity, increased thermalactivity and processivity

smART Reverse Transcriptase
E1376-02	EURx	4x10000U	EUR 258.33
Description: Recombinant reverse transcriptase with reduced RNase H activity, increased thermalactivity and processivity

THERMOscript H Minus M-MuLV Reverse Transcriptase (thermostable reverse transcriptase)
MBS355984-10KUnits	MyBiosource	10KUnits	EUR 315

THERMOscript H Minus M-MuLV Reverse Transcriptase (thermostable reverse transcriptase)
MBS355984-5x10KUnits	MyBiosource	5x10KUnits	EUR 870

Reverse Transcriptase Enzyme
abx461074-1096tests	Abbexa	10 × 96 tests	Ask for price

Reverse Transcriptase Enzyme
abx461074-596tests	Abbexa	5 × 96 tests	Ask for price

Reverse Transcriptase Enzyme
abx461074-96tests	Abbexa	96 tests	EUR 525

SCRIPT Reverse Transcriptase
PCR-425-100KU	Jena Bioscience GmbH	100kilo units	EUR 655

SCRIPT Reverse Transcriptase
PCR-425-10MU	Jena Bioscience GmbH	10 mio units	Ask for price

SCRIPT Reverse Transcriptase
PCR-425-1MU	Jena Bioscience GmbH	1Mio units	EUR 4674.05

SCRIPT Reverse Transcriptase
PCR-505L	Jena Bioscience GmbH	100000units	EUR 679.87

SCRIPT Reverse Transcriptase
PCR-505S	Jena Bioscience GmbH	20000units	EUR 170.02

BioReady I Reverse Transcriptase
TRI-A46M1	TRI Biotech	50000U（250T）	EUR 2199.96

BioReady I Reverse Transcriptase
TRI-A46S1	TRI Biotech	10000U（50T）	EUR 478.8

Telomerase reverse transcriptase
AP79299	SAB	1mg	EUR 2640

Telomerase reverse transcriptase
AP88607	SAB	1mg	EUR 2640

Telomerase reverse transcriptase
AP88646	SAB	1mg	EUR 2640

Telomerase reverse transcriptase
AP88990	SAB	1mg	EUR 2640

Telomerase reverse transcriptase
AP89047	SAB	1mg	EUR 2640

Telomerase reverse transcriptase
E8ET1702-22	EnoGene	100ul	EUR 275
Description: Available in various conjugation types.

Telomerase reverse transcriptase
MBS8534331-01mL	MyBiosource	0.1mL	EUR 345

Telomerase reverse transcriptase
MBS8534331-01mLAF405L	MyBiosource	0.1mL(AF405L)	EUR 565

Telomerase reverse transcriptase
MBS8534331-01mLAF405S	MyBiosource	0.1mL(AF405S)	EUR 565

Telomerase reverse transcriptase
MBS8534331-01mLAF610	MyBiosource	0.1mL(AF610)	EUR 565

Telomerase reverse transcriptase
MBS8534331-01mLAF635	MyBiosource	0.1mL(AF635)	EUR 565